《马力欧+疯兔 星耀之愿》的可用角色包括马力欧、桃花公主、路易吉，和他们的疯兔版，以及新加入的疯兔罗洁塔，酷霸王和神秘角色 Edge。这其中少了前作《马力欧+疯兔 王国之战》的耀西。对于耀西缺席的原因，《马力欧+疯兔 星耀之愿》制作人 Xavier Manzanares 在 Nintendo Everything 的采访中表示，这是因为他们“不想再次做同样的事情”。Manzanares 提到，《星耀之愿》并不是定位为《王国之战》的直接续集，所以比起直接沿用《王国之战》中的角色，他们相信玩家更希望角色和其能力是合乎故事逻辑的。由此他们打开了思路，设计了新的神秘角色 Edge。Edge占了挺大一块空间看起来新角色 Edge 会和故事有很大的关联，因为 Manzanares 说：“我不能谈论更多关于她的过去和她在故事
Available characters in Mario + Crazy Rabbit star wish include Mario, peach blossom princess, Luigi, and their Crazy Rabbit version, as well as the newly added Crazy Rabbit Luo jieta, cool overlord and mysterious character edge. There is no Yao Xi, who previously wrote the battle of Mario + mad rabbit kingdom. For the reason why Yao Xi was absent, Xavier Manzanares, the producer of "Mario + Crazy Rabbit star wish", said in an interview with Nintendo everything that this was because they "didn't want to do the same thing again". Manzanares mentioned that "the wish of the stars" is not positioned as a direct sequel to "the battle of the kingdom", so they believe that players prefer to follow the characters and their abilities in "the battle of the kingdom" rather than directly follow the characters in "the battle of the kingdom". Therefore, they opened their minds and designed a new mysterious character edge. Edge takes up a lot of space. It seems that the new character edge will have a lot to do with the story, because Manzanares said, "I can't talk more about her past and her story
